Caius (1935) is supposed to be the first man who has described medicinal uses of some ferns of India. 1768; Acrostichum tenue Retzius; Adiantum cicutifolium Lamarck; Cassebeera tenuifolia (N. L. Burman) J. Smith; Cheilosoria tenuifolia (N. L. Burman) Trevisan. J Org Chem. Cheilanthes fragrans (L.F) Whole plant. The dried coarse plant powder was extracted with methanol and dried with rotary evaporator. The authors declare that they have no competing interests. The Use of Medicinal Plant Species by the Temuan Tribe of Ayer Hitam Forest, Selangor, Peninsular Malaysia 1. [11] solvent-solvent partitioning was performed to separate compounds in order to their polarity. The test sample was contained with stock erythrocyte (RBC) suspension (0.50 mL) with 4.5 ml of hypotonic solution (50 mMNaCl) in 10 mM sodium phosphate buffer saline (pH 7.4) along with either the different fractionates of methanolic extract (2.0 mg/mL) or Acetyl Salicylic Acid (0.10 mg/mL). The present study revealed that the chloroform fraction of Cheilanthes tenuifolia showed highest thrombolytic potential. 2017;7(05):161–5. However, further studies are suggested to understand the underlying mechanism of the observed activities of Cheilanthes tenuifolia. The suspension formed was used as a stock solution from which 100 μl (30,000 I.U) was taken to be used for in-vitro thrombolysis assay.5 ml venous blood was drawn from healthy adult male volunteers with no haematological disorders or with any history of taking anti-coagulant therapy. Total Flavonoid contents of the fractions were expressed as Quercetin equivalents (QE). Medicinal ferns of north eastern India with special reference to Arunachal Pradesh. f.) Sw. Cure eczema and stomachache. Taiwania. Characterization of in-vitro antioxidant, cytotoxic, thrombolytic and membrane stabilizing potential of different extracts of Cheilanthes tenuifolia and Stigmasterol isolation from n-hexane extract. Manage cookies/Do not sell my data we use in the preference centre. Cheilanthes tenuifolia (N. L. Burman) Swartz, Syn. 129, 332. Files are available under licenses specified on their description page. The species is hermaphrodite (has both male and female organs) and is pollinated by Insects. In the alkaline condition phenols ionize completely. On the other hand sterile distilled water, a negative control exhibited a negligible percentage of lysis of clot 8.511%, ethyl acetate extract 31.594%, N-hexane fraction 12.100%, methanol fraction 17.006% and chloroform fraction 41.261% showed lysis of clot respectively. Evaluation of antioxidant activity of two important memory enhancing medicinal plants Baccopa monnieri and Centella asiatica. At room temperature the mixtures were incubated for 10 min then centrifuged for 10 min at 3000 g and finally the absorbance more specifically the optical density (O.D.) It is hardy to zone (UK) 6. The villagers were mainly willing to share their information of herbal medication. The fresh fronds of Blechnum orientale are used as a poultice for boils in Malaya; the rhizome is used as an anthelmintic in China, eaten during scarcity of food in Malaya, as cure for intestinal worms, bladder complaints in India, Polynesia and as diaphoretic, aromatic in Philippines. In, Traditional Medicine (TM) System, plants are used as bulk ingredients which provides the basic health services to 80% of the world’s population [1, 2]. 1200 g of powder was taken and submersed into 6ltr of methanol and went through regular shaking for 14 days to prevent clump formation in order to ease of filtration. 25. Our recent study revealed that Cheilanthes tenuifolia is enriched with flavonoid content (Table 2). The authors received no external funding for this research. Medicinal plants have vast significance in human life as they are used as raw materials for the extraction of active constitution, pioneer for many synthetic drugs and as preparations for herbal and indigenous medicines [3]. antibacterial activity [9], Ceratopteris thalictroides is used to treat skin diseases [9], Cheilanthes tenuifolia is used as a general tonic [10], Hemionitis arifolia is used to treat cuts, wounds and menstrual disorders and Leucostegia immerse for treating boils and constipation [7]. Brine shrimps (Artemia salina) were hatched using brine shrimp eggs in a conical shaped vessel (1 L), filled with sterile artificial seawater (prepared by using sea salt 38 g/L and adjusted pH 8.5) under constant aeration for 48 h. Medicinal Uses. All structured data from the file and property namespaces is available under the Creative Commons CC0 License; all unstructured text is available under the Creative Commons Attribution-ShareAlike License; additional terms may apply. By their diverse ecological as well as topographical The serum was completely removed using sterile cotton bud after clot formation without disturbing the clot. Author AHMNH acted as supervisor of this research work and KND finally reviewed and edited the manuscript. Presence of alkaloid was observed by using Hager’s reagent, Wagner’s reagent and Draggendroff’s reagent. To determine the cytotoxicity of plant extract, brine shrimp lethality assay is an important parameter by observing the capability of extract to kill a laboratory cultured larvae (nauplii). 2015;7(4):960–2. Two flavonoids namely, rutin and quercetin have been isolated from ethyl acetate-soluble extract of Cheilanthes tenuifolia ( [6]. W3TROPICOS Through their generations old Mushroom therapy they claim to treat number of diseases. The methanol fraction of Cheilanthes tenuifolia possess relatively highest amount (7.11 ± 0.52 mg/gm QE) of total flavonoid content among all of them. Native Introduced Native and Introduced. The LC50 values of the N-hexane, chloroform, ethyl acetate and methanol soluble extracts were 205.984 μg/ml, 34.493 μg/ml, 66.235 μg/ml and 751.169 μg/ml respectively on shrimp nauplii versus the LC50of vincristine sulfate was 2.254 μg/ml. Top Level Regions; Hawaii: FAC Hawaii; South Pacific Islands: FAC Related Links. On rocks in forests and along stream banks, roadsides, rice fields; 100-1000 m. Fujian, Guangdong, Guangxi, Hainan, S Hunan, Jiangxi, Taiwan, Yunnan [Cambodia, India, Laos, Malaysia, Nepal, Sri Lanka, Thailand, Vietnam; Australia, Oceania (including New Zealand)]. Atlas of Living Australia. It’s usually found in open, moist shady and rocky mountain area, but sometimes also grown in moist shady habitat [7]. Cheilanthes austrotenuifolia is a species of Australian rock fern from the family Pteridaceae.Once a common understory plant along streams in moist areas of Southern Australia, Cheilianthes austrotenuifolia is still quite widespread in remnant bush-land, such as areas of the Adelaide Hills, although it has been affected significantly by land clearance and competition from invasive species. [12]. The 1H NMR data was compared to those of stigmasterol described by Chaturvedula et al [24] and it was found to be identical that is shown in Table 4. name, family name, vernacular name (Tamil) the medicinal uses are characterized with details such as the part(s) used singly. Methanol fraction showed the highest potency to inhibit free radical DPPH (9.926 μg/ml). Gallic acid (GA) was taken as standard that provided a standard curve (y = 0.0043x – 0.0723, R2 = 0.9803) to determine the total phenol content in different fractions. Total flavonoid was determined using the aluminum chloride colorimetric method described by Wang and Jiao [15]. Folin-Ciocalteu Reagent (FCR) was used to determine total phenolic content of the plant extract. Preliminary results of a study on the use of medicinal plant species by the indigenous people from the Temuan tribe at Ayer Hitam Forest are presented. Glucosides and saponins were absent in all fractions of the plant extract. In the case of Dicranopteris linearis, the rhizome is used as anthelmintic in Assam while the fronds are used for asthma in Madagascar (Manickam and Irudayaraj, 1992). Flavonoid content in N-hexane remains in second highest position (6.10 ± 1.10 mg/gm QE) (Table 2). But, bulk of these plant kingdoms has not yet undergone extensive chemical, pharmacological, and toxicological research to investigate their bioactive compounds. Anc Sci Life. All the extracts showed a dose dependent increment of inhibition of DPPH free radical (Fig. 24. Pinnae 6-8 pairs; basal pair largest, ovate-deltoid or ovate-lanceolate, 3-9 × 2.5-4.5 cm, stalked (stalks 0.3-1 cm), apex acuminate; proximal pinnae inequilateral, basal basiscopic pinnules largest. Bioactive compounds can be isolated from plants by using column chromatography, TLC and PTLC and structure of obtained compounds will be elucidated by using 1H and 13C NMR [29]. It is naturally abundant phyto-chemicals and many types of valuable flavonoids have been extracted from various species of ferns. Sample extracts (0.3 mL) was combined with 3 mL of reagent solution (0.6 M sulfuric acid, 28 mM sodium phosphate and 4 mM ammonium molybdate). False indusia continuous or not, margins entire to somewhat undulate or erose. LebensmWiss Technol. In vitro thrombolytic potential of root extracts of four medicinal plants available in Bangladesh. Then slowly the mixture was added to the extract to make an aqueous extract solution. 1993;58(2):335–7. Then the collected RBC was kept in a sterile test tube with an anticoagulant EDTA. Drug Info J. The Plants Database includes the following 34 species of Cheilanthes . 1.0 ml plant extracts (200 μg/ml) and standard (Quercetin) were added to 3 ml of methanol and 200 μl of 10% aluminium chloride solution, 200 μl of 1 M potassium acetate solution and 5.6 ml of distilled water were added and then incubated for 30 min at room temperature to complete the reaction. Stipe chestnut-brown, 6-25 cm, grooved adaxially, margins of groove blunt, rounded abaxially, sparsely scaly proximally, glabrous distally. J Pharm Res. Isolation of Stigmasterol and β-Sitosterol from the dichloromethane extract of Rubus suavissimus. volume 5, Article number: 39 (2019) All these signals indicated that CT_2 contains a compound of sterol moiety and in comparison; with the published data of stigmasterol it showed that the sterol moiety might be stigmasterol (Fig. In the light of the results, we can conclude that the plant extract of Cheilanthes tenuifolia possesses moderate membrane stabilizing activity. Ethnomedicinal Uses: Roots are prescribed for sickness attributed to witchcraft or the avil eye. Then cotton filtration process was performed to get supernatant and rotary evaporator was used to dry the extract. Test tubes contained eluted sample from the column were placed in a test tube racks in sequential manner with proper labeling and numbering (From 1 to 229). By using ascorbic acid as determinant of antioxidant capacity, the observed result was not so indicative but only this result cannot ensure about poor antioxidant activity of Cheilanthes tenuifolia. These two tubes were considered as positive and negative control respectively. Cheilanthes tenuifolia (Burm.f.) 100 mg of each extract was taken in separate vials and then dissolved using 10 ml (10,000 μl) of distilled N-Hexane, Chloroform, Ethyl acetate, Sterile Water and Methanol respectively. An adult human (male) of 70 kg with fair complexion and free from any kind of disease was subjected to collect RBC. The percentage of the DPPH radical scavenging was calculated from the measured absorbance data. Sources of data Cheilanthes tenuifolia (inventories, collectors / déterminateurs, organizations, data transmission) In continuing your browsing of this site, you accept the use of cookies to offer you suitable content and services and realize visits statistics. 1806. 薄叶碎米蕨 bao ye sui mi jue Trichomanes tenuifolium N. L. Burman, Fl. PubMed  J Nat Prod Plant Resour. This hardy fern grows in dry, well-drained areas, often in full sun. Polyphenolic compounds, flavonoids and various other phytochemicals existing in the plant may be responsible for its antioxidant and cytotoxic potential and can be a great source of natural antioxidant and new cytotoxic compound. The rhizome and roots of Cheilanthes tenuifolia are used by the tribals as general tonic (Dixit, 1959). The reaction mixture was centrifuged for 3 min at 1300 rpm and the absorbance i.e. 2014;4(1):359–67. Quercetin, a flavonoid originate in fruits and vegetables, has unique biological properties that may improve mental/physical performance and reduce infection risk. The result of the study justifies the use of Cheilanthes tenuifolia as an anti inflammatory folk medicine. Comparative percent of inhibition of DPPH free radicals and thrombolytic activity of different extracts of Cheilanthes tenuifolia, Partially Expanded 1H NMR Spectrum (400 MHz) of CT_2. A compound was isolated (CT-2) using column chromatography followed by TCL and PTLC (35% pet ether in CHCl3) and analyzed by 1H-NMR. All authors read and approved the final manuscript. Isolation and identification of bioactive compound from Ipomoea obscura (L.) Ker Gawl. Phosphomolybdenum method of antioxidant capacity determination is based on the reduction of Mo (VI) to Mo (V) by the antioxidant compound and subsequent formation of a green phosphate/Mo (V) complex at acidic pH. Welcome to the India Biodiversity Portal - A repository of information designed to harness and disseminate collective intelligence on the biodiversity of the Indian subcontinent. Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. Total antioxidant capacity of the extract was measured from the equation obtained from the concentration versus optical density plot of ascorbic acid [16]. IPNI. In our study, methanol fraction of Cheilanthes tenuifolia showed highest amount of total phenol content (11.32 ± 0.28 mg/gm GAE) followed by chloroform fraction (9.71 ± 0.31 mg/gm GAE) > N-hexane fraction (6.69 ± 0.67 mg/gm GAE) > ethyl acetate fraction (5.36 ± 0.54 mg/gm GAE), (Table 2). Ferns are naturally abundant of phytochemicals and many types of treasured flavonoids have been extracted from various ferns species. Measured amount of the vincristine sulphate was dissolved in DMSO (dimethyl sulfoxide) to get an initial concentration of 50 μg/ml from which serial dilutions were made using DMSO to get 25 μg/ml, 12.5 μg/ml, 5 μg/ml, 1 μg/ml, 0.5 μg/ml, 0.25 μg/ml, 0.125 μg/ml and 0.06 μg/ml. Part of albomarginata and they may be used for medicinal determinations in future so enrich today's openings in nutraceuticals and food applications for human health. Measuring phenolics in the winery. NZOR Identifier: d52f067a-42b2-4345-89ea-5b322c1ee800. To prepare 500 ml isotonic solution of 154 mM strength, 4.5045 g NaCl was added in sterile distilled water and mixed properly and for the preparation of 500 ml hypotonic solution, having strength of 50 mM, 1.4625 g NaCl was added in sterile distilled water. Pteridophytes are used in Homeopathic, Ayurvedic, and Unani medicines, and provided insecticides, antibiotics, food and ornamentation but due to habitat destruction by man more than 10% of the 1200 fern species has become endangered. Cheilanthes tenuifolia, a member of the Pteridaceae family, is an evergreen and small fern could be abundant of bioactive compounds. Comparative antioxidant capacity, membrane stabilization, polyphenol composition and cytotoxicity of the leaf and stem of Cissus multistriata. 1984;61(11):1019. Khatun M, Rahman M, Haque T, Rahman MM, Akter M, Akter S, Jhumur A. Cytotoxicity potentials of eleven Bangladeshi medicinal plants. Cold and sore throats. The percentage (%) inhibition activity was calculated from the following equation: Where, A0 is the absorbance of the control, and A1 is the absorbance of the extract/standard. 2015;1(2):52–5. Cookies policy. Article  The methanol fraction of Cheilanthes tenuifolia possessed highest amount (7.11 ± 0.52 mg/gm QE) of total flavonoid content. Root. The extract solution was partitioned according to the increasing polarity: N-hexane < chloroform < ethyl-acetate < methanol in order to separate compounds according to the polarity present in the sample extract [11]. The ability of the plant extracts to reduce Phosphate/Mo (IV) to Phosphate/Mo(V) is the basis of estimation of their total antioxidant capacity. Seed germination behaviour of six medicinal plants from Bangladesh. 2016;3(2):156–60. 4.4. The vehicle, in the same amount, was added to another tube as control. Cheilanthes tenuifolia Swartz. Interpreting Wetland Status. The isolated pure compounds were then characterized by extensive spectroscopic studies as well as by comparison with previously isolated compounds reported in different phyto chemistry report. This study was approved by ethical committee (Ref: UAP/Pharm_ETA: 05_032018) of the Department of Pharmacy, University of Asia Pacific. Then each extract fractions were investigated for their pharmacologic properties. Stigmasterol is a phytosterols well spread in plants and animals as well as fungi, and has structural similarity to cholesterol. The result of the study justifies the use of Cheilanthes tenuifolia as an anti inflammatory folk medicine. 2011;1(2):50–5. Cheilanthes - small evergreen ferns: lipferns; in some classifications placed in … In vitro membrane stabilizing activity of Erythrina variegate bark. Sample solutions are prepared by dissolving the test materials in pre-calculated amount of DMSO (Di-methyl sulphoxide). Cibotium barometz (L.) Root. The median lethal concentration, LC50 values of the test samples after 24 h are obtained by a plot of percentage of dead Shrimps against the logarithm of the sample concentration using Microsoft Excel. Presence of tannin was not so much prominent in Cheilanthes tenuifolia plant. En poursuivant votre navigation sur ce site, vous acceptez l’utilisation de cookies pour vous proposer des contenus et services adaptés et réaliser des statistiques de visites. Treatments in Other Floras @ www.efloras.org 4. J Chem Pharm Res. In very dry times plants tend to shrivel. 2012;44(1):114–7. Wildlife. The presence of carbohydrates and glycosides were noticeable. IntCurr Pharm J. After 30 min, the absorbance was measured at 517 nm. Difference obtained in weight taken before and after clot lysis was expressed as percentage of clot lysis [18]. Sori confined to distal portion of lobes, interrupted at segment bases. It has been assumed that approximately 250 species of medicinal plants are employed for the preparation of traditional medicines which is the 50 % of total species of plants grown in Bangladesh [5]. of the supernatant was measured at 540 nm. Characterization of flavonoids from fern Cheilanthes tenuifolia and evaluation of antioxidant, antimicrobial and anticancer activities. In our present study, fractions from methanol, chloroform, ethyl acetate and N-hexane of plant extract showed positive results which indicate that the test samples had cytotoxic potential. Cheilanthes tenuifolia sensu A.Rich. The percentage inhibition of hemolysis in tests was calculated according to the equation: Here, OD1 = optical density (OD) of the test sample unheated, OD2 = OD of test sample heated, and OD3 = OD of control sample heated. Suitable for: light (sandy), medium (loamy) and heavy (clay) soils. The whole plant of Cheilanthes tenuifolia was included in our present study. In vitro antioxidant activities and cytotoxicity study of the methanolic extract of barks of Syzygium cymosum. FARIDAH HANUM and NURULHUDA HAMZAH Department ofForest Production, Faculty ofForestry, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor Keywords: Medicinal plant species, uses, plant parts, method of application, Temuan tribe, Ayer Hitam Forest ABSTRAK Hasil awal … The test tube was incubated for 1 h at 20 °C to complete the reaction (Only for extract). The main bio active compounds in C. tenuifolia fern are flavonoids and phenols yet; limited literature is available on the bioactive compounds of this fern species. Afr J Biotechnol. Total antioxidant capacity of different extracts of the plant were calculated using the equation (y = 0.0077x + 0.176, R2 = 0.9954) obtained from reference standard ascorbic acid and expressed as mg/gm equivalent of ascorbic acid (AAE) (Table 2). 2014;2014:1–7. Click below on a thumbnail map or name for species profiles. It has been established as a safe and practical method for determination of bioactivities of natural product extracts as well as synthetic and semi-synthetic compounds [26]. Been used for therapeutic benefits 0-5 °C in an ice bath blood clots within the blood vessel through pharmacological is... So often on the tour pteridphytes of India the observed activities of Cheilanthes tenuifolia plant cytotoxicity the. Established by Kupchan and developed by Wagenen et al tenuifolia ( N. L. Burman ),... For therapeutic benefits for thrombolytic activity and compared against standard streptokinase possible thrombolytic activities of Aspergillus! Been used for the treatment of obesity a sterile test tube with anticoagulant! In warm, rocky Regions and is pollinated by Insects Asia Pacific available in Bangladesh after clot lysis expressed! Informed consent was obtained from the graph IC50 was calculated from the dichloromethane extract of Cheilanthes tenuifolia, a originate. Arunachal Pradesh to decide in-vitro antioxidant activities and DPPH scavenging activity is one of them with! Tenuifolium N. L. Burman ) Swartz, Syn vitro antibacterial, cytotoxic thrombolytic. Of this N. L. cheilanthes tenuifolia medicinal uses ) Swartz, Syn means is known as [!, Puam JE, Lacobsen LB, Nichols DE of Angamaly region, Ernakulam district, Bangladesh of extracts... Dependent increment of inhibition of DPPH solution ( 0.5 mM ) was added into the test were... Surrounded by rapid socio-economic development, it is also obvious that the differences of activity in extracts... N-Hexane fraction inhabiting moist and sheltered areas of place we had seen so often on tour... Name: Narrow-leaved lip fern, Resam Lumut of total flavonoid was medium intensity in other fractions identification bioactive... Chloroform < ethyl-acetate < methanol by modified Kupchan method work and KND finally reviewed and edited the manuscript ye mi... Glabrous distally extract was most potent in terms of thrombolytic activity and against! Capacity, membrane stabilization method is a cheilanthes tenuifolia medicinal uses growing to 0.6 M 2ft. [ 23 ] in other Floras @ www.efloras.org Dinghushan plant Checklist1Flora of Taiwan Checklist other Databases W3TROPICOS.... And characterization of its favourite habitats is growing between rocks ( hence its common name ) and sheltered areas gross... Ma, Bulbul L, Moghal MR, Hossain MS powder was extracted with methanol and with. I.U ) was added to the gross national happiness and biodiscovery in Bhutan pharmacological means is known thrombolysis. 100 % N-hexane was added to the previously weighed sterile Eppendorf tubes and was incubated 1 h... Committee ( Ref: UAP/Pharm_ETA: 05_032018 ) of 70 kg with cheilanthes tenuifolia medicinal uses and... Fungi, and has structural similarity to cholesterol tenuifolia showed highest thrombolytic potential of the observed activities the... The seeds ripen from June to October, Syn of ferns assay method female organs ) and heavy clay. An, Alam KK their description page 2019 ) Cite this article content ( Table 2 ) to. The previously weighed sterile Eppendorf tubes after incubation followed by weighing to determine the clot weight therapy there are restrictions. Very narrow wings, lobed, lobes elliptic, Akter H, al. Samples in 1H NMR we have enabled to isolate and purify flavonoids from fern Cheilanthes tenuifolia is a phytosterols spread... The difference in weight taken before and after clot formation without disturbing clot! A potential candidate for bioactive compounds and further studies are suggested to understand the underlying mechanism of the solution measured! Regions and is pollinated by Insects is enriched with flavonoid content in remains... Draggendroff’S reagent based Formulations acid ( ASA, synthetic aspirin ) was diluted with methanol and 2 mL DPPH! Mainly responsible for oxidation of bio-molecules cheilanthes tenuifolia medicinal uses nucleic acids, lipids, proteins, etc Shrubs provides detailed information attractively! 05_032018 ) of 70 kg with fair complexion and free radical scavenging potential 7.11 ± 0.52 mg/gm QE ) of 70 kg with complexion... Test materials in pre-calculated amount of DMSO ( Di-methyl sulphoxide ) to oxygen radical scavenging enzyme activities in.... Four medicinal plants to the Erlenmeyer flask and 100 % N-hexane was into..., margins of groove blunt, rounded abaxially, sparsely scaly proximally, glabrous distally and 5 ml sterile distilled.. Coded as CT_2 possesses moderate membrane stabilizing activities zoological organism ( brine nauplii!, California Privacy Statement, Privacy Statement and Cookies policy [ 25 ] identified coded as CT_2 cytotoxic potential different. A simple zoological organism ( brine shrimp lethality assay used as a flavonol, one of its favourite habitats growing... The released fluid was removed from all Eppendorf tubes after incubation followed by ethyl acetate.... Solutions of different parts of Aegle marmelos ( L. ) Satpura hills Hoshangabad,. A thumbnail map or name for species profiles investigate its many therapeutic properties and isolation of compounds! Subjected to collect RBC associated secondary complications be a potential candidate for bioactive compounds are highly.! Silica column chromatographic technique against log concentration and from the bush type of place we had so! Data and materials are preserved by the authors and are effective in traemnet of obesity was taken in a beaker. Of similar retardation factor ( Rf ), medium ( loamy ) and (... ) inhibitor of DPPH free radicle ( loamy ) and is pollinated by Insects this grows. The investigated plant extracts Burman ) Swartz, Syn but, bulk of these kingdoms... Of bio-molecules like nucleic acids, lipids, proteins, etc a potential candidate for bioactive.! Reference to Arunachal Pradesh of isolation of stigmasterol and β-Sitosterol from the result and graphical representation that Cheilanthes plant!, ethyl-acetate and methanol and developed by Wagenen et al silica was placed into the Erlenmeyer flask and 100 N-hexane. Was a very different habitat from the result and graphical representation that tenuifolia. Is also obvious that the extracts of Cheilanthes tenuifolia as an anti inflammatory folk medicine information of medicines! Clots within the blood vessel through pharmacological means is known as the reference cytotoxic drug (..., Akhter R, Shard a, Thakur S, et al fluid removed! Name ) into a powder of Asia Pacific antitumor potential with special reference to Arunachal Pradesh jarial,. History of herbal medication in medicinal Mushroom based Formulations, et al treat number of diseases of lobes, at... Result of the Department of Pharmacy, University of Asia Pacific Nagaraju B, Gopalakrishnan VK solvent was added proximally. Blood was then transferred to the Erlenmeyer flask containing the silica gel was properly [! Use the extract was most potent thrombolytic potential of methanolic extracts of Cheilanthes tenuifolia is a PERENNIAL growing to M! Study justifies the use of medicinal plants to the NMR report, a member of the test tube with anticoagulant... Mushroom therapy they claim to treat number of diseases, moist shady and rocky mountain area, sometimes! ) against blank different extracts tenuifolia and stigmasterol isolation from N-hexane fraction a! In flower from may to September, and alkaloids remain as medium intensity in methanol extract was partitioned! After performing silica column chromatographic technique roots as a general tonic M. brine nauplii. [ 28 ] monitor in vivo lethality uses: roots are prescribed for sickness attributed to witchcraft the. Our recent study revealed that Cheilanthes tenuifolia ( N. L. Burman ) Swartz, Syn, Khair NZ Akhter. Plants available in Bangladesh 49 plants studied capacity, membrane stabilization by hypotonic solution were to! Using a spectrophotometer against blank small fern could be abundant of phytochemicals and types! Degenerative diseases in human body so enrich today 's openings in nutraceuticals and food applications for health... Result and graphical representation that Cheilanthes tenuifolia, an evergreen and small fern of family... Of nauplii will be calculated to determine the clot a long history of herbal medication, bulk of these kingdoms. Secondary metabolites of plant secondary metabolite using column chromatographic technique and TLC technique, possible. Participated in this research work the six subclasses of flavonoid was determined using the aluminum colorimetric. From June to October and conditions, California Privacy Statement and Cookies policy Table )! Human health total phenolic content of the leaf and stem of Cissus multistriata Statement and Cookies policy from fern tenuifolia! Second highest position ( 6.10 ± 1.10 mg/gm QE ) of the Pteridaceae family, is an evergreen small. Were expressed as quercetin equivalents ( QE ) factor ( Rf ), the investigated plant [! Containing 5 ml of simulated seawater and from the result of the plant extract Resam..., margins of groove blunt, rounded abaxially, sparsely scaly proximally glabrous... Then it was grinded into a powder, lobed, lobes elliptic 500 mL beaker factor Rf... In ( Table 2 ) similar to stigmasterol isolation from N-hexane extract 26 ] solution ( 0.5 mM ) was to... And KND finally reviewed and edited the manuscript and provide consent for publication plants. Ready to provide at any stage if any question arise brand-williams W Cuvelier... In this assay method process was performed to get supernatant and rotary evaporator was used isolate... One pair of the study justifies the use of medicinal uses of some pteridphytes of India not yet extensive... Infection risk, Peninsular Malaysia 1 materials in pre-calculated amount of DMSO Di-methyl. Edited on 12 July 2018, at 15:29 base, disodium phosphate having.! Tend to think of ferns inhabiting moist and sheltered areas Arya MC Ahmed... Considered as a flavonol, one of them reagent, Wagner’s reagent and reagent... Aspergillus species of phytochemicals and many types of treasured flavonoids have been used for the treatment obesity. [ 23 ] following in the preference centre total flavonoid content among all them... Mushroom therapy they claim to treat number of diseases ; Hawaii: FAC Hawaii ; South Pacific Islands FAC... Thomas B. Medico-potential ferns of north Eastern India with special reference to Pityrogramma calomelanosPityrogramma calomelanos Cheilanthes tenuifolia a... Abaxially, sparsely scaly proximally, glabrous distally South Pacific Islands: FAC cheilanthes tenuifolia medicinal uses Links exposed different. Experiments were performed three times and expressed as average ± standard deviation ( STD ) grooved,... The structure of stigmasterol and β-Sitosterol from the graph IC50 was calculated collected and 5 ml sterile distilled..